The OF·27 protocol is a fixed-dose topical regimen. The formulation is supplied in a 50 millilitre airless pump that delivers a metered dose with each press. The protocol specifies one application in the morning and one in the evening, two metered doses a day, and one bottle is a four-week cycle.
Each dose is applied directly to the scalp, parting the hair so the formulation reaches the skin rather than the hair shaft, and then worked in with the fingertips for roughly thirty seconds at the point of application. This step matters. The follicle and its growth-factor receptors sit in the skin, not on the fibre; the brief massage distributes the metered dose across the application area and brings it into contact with the scalp, which is where the signalling described in the earlier notes takes place. A dose left on the hair is a dose that does not reach its target.
Several details of the format follow from the science in the earlier notes. The container is airless, which limits the exposure of the proteins to the air and light that the stability discussion in Note 03 identifies as a concern. The dose is metered rather than free-poured, so each application is the same quantity, which is the condition under which the study in Note 09 was conducted. The pump engine is metal-free, chosen to avoid contact between the formulation and metal components across a cycle.
The protocol is twice daily and continuous because the mechanism it supports is the follicle's growth phase, which operates over weeks rather than from a single application. The twelve-week span of the study in Note 09 corresponds to three consecutive cycles of the protocol.
This note documents how the formulation is used. What a full cycle, and the weeks beyond it, correspond to in the study record is the subject of the final note in this group.